Autologous Serum Skin Test in Chronic Urticaria

Author Details : Mukunda Ranga Swaroop, Belagola Dasegowda Sathyanarayana, Ankita Gupta, Aneesa, Priyanka Kumari, Ji

Volume : 1, Issue : 1, Year : 2015

Article Page : 25-27

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Background: Chronic Urticaria (CU) is a common dermatosis affecting 0.1% population, characterized by recurrent appearance of wheals with or without angioedema for more than 6 weeks. However, in a significant number of these patients, chronic autoimmune urticaria has been identified. Autologous serum skin test (ASST) is a useful diagnostic aid in identifying chronic autoimmune urticaria.
Aim: To assess autologous serum skin test in chronic urticaria.
Methods: A total of 50 patients with chronic urticaria were taken up for the study and ASST was performed in all the patients. Approximately 0.05 ml (equivalent to 2 units on insulin syringe that has 1 ml marked as 40 units) of autologous serum was injected intradermally over flexor aspect of the left forearm. Equal amount of normal saline (negative control) was injected intradermally over flexor aspect of the right forearm. The results were read after 30 minutes. The test was considered positive if wheal and flare response occurred over the left forearm (serum injection site) with a diameter of at least 1.5 mm or more than that of saline induced response.
Results: Out of 50 patients with chronic urticarial, 30 (60%) were autologous serum skin test positive whereas 20 (40%) were autologous serum skin test negative.
Conclusion: Positive autologous serum skin test was seen in 60% of patients. It is a simple, practicable in-vivo intradermal clinical test to differentiate chronic idiopathic urticaria from autoimmune urticaria.

Key Words:
Chronic urticaria, Chronic autoimmune urticaria, Autologous serum skin test, In- vivo intradermal test

How to cite : Swaroop M R, Sathyanarayana B D, Gupta A, Aneesa, Kumari P, Ji, Autologous Serum Skin Test in Chronic Urticaria. IP Indian J Clin Exp Dermatol 2015;1(1):25-27

Copyright © 2015 by author(s) and IP Indian J Clin Exp Dermatol. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (

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